TABLE 2

Intra- and interday precision and accuracy for LC-MS/MS quantification of P450 probe drugs and corresponding metabolites in mouse plasma

Concentrations are given in nanomoles. Values represent means ± S.D. or percentages unless otherwise indicated.

Analyte	Nominal  Concentration	Intra-assay (n = 3)			Interassay (n = 9)
Analyte	Nominal  Concentration	Measured  Concentration	Relevant S.D.	Accuracy	Measured  Concentration	Relevant S.D.	Accuracy
PHE	75	69.93 ± 18.70	26.7	93.2	75.69 ± 10.84	14.3	101
	400	387.7 ± 20.8	5.38	96.8	394.9 ± 15.4	3.89	98.6
	4000	3580 ± 114	3.17	89.4	3707 ± 168	4.54	92.6
APAP	15	14.97 ± 1.85	12.4	99.9	15.69 ± 1.23	7.84	104
	80	81.57 ± 0.96	1.18	102	78.96 ± 2.38	3.02	98.7
	800	699.7 ± 11.8	1.69	87.4	722.6 ± 36.5	5.05	90.3
MDZ	15	15.40 ± 1.95	12.7	103	14.63 ± 1.98	13.6	97.5
	80	73.77 ± 5.13	6.95	92.2	77.96 ± 9.29	11.9	97.4
	800	688.7 ± 66.3	9.62	86.1	721.3 ± 57.2	7.93	90.2
1′-OH-MDZ	75	71.47 ± 3.50	4.90	95.4	74.56 ± 6.18	8.29	99.4
	400	382.3 ± 23.0	6.02	95.6	378.0 ± 14.4	3.80	94.5
	4000	3523 ± 136	3.86	88.1	3661 ± 272	7.42	91.6
DXM	75	64.67 ± 10.80	16.7	83.0	66.46 ± 7.47	11.2	87.5
	80	356.0 ± 14.7	4.14	89.0	371.8 ± 19.6	5.27	92.9
	800	3767 ± 186	4.93	94.2	3741 ± 161	4.29	93.6
DXO	15	12.67 ± 2.63	20.7	84.6	12.87 ± 2.14	16.7	85.8
	400	67.90 ± 4.48	6.60	84.9	72.92 ± 5.45	7.48	91.1
	4000	700.0 ± 25.9	3.71	87.5	736.8 ± 39.4	5.35	92.1
DIC	75	86.20 ± 6.77	7.86	115	90.33 ± 21.56	23.9	121
	400	468.3 ± 11.2	2.40	117	421.8 ± 48.6	11.5	106
	4000	3647 ± 123	3.38	91.1	4148 ± 604	14.6	104
4′-OH-DIC	15	15.70 ± 2.48	15.8	105	17.61 ± 3.02	17.2	118
	80	86.87 ± 15.76	18.2	109	81.39 ± 12.65	15.6	102
	800	826.3 ± 20.2	2.45	103	876.6 ± 102.8	11.7	110
CLZ	75	87.60 ± 8.88	10.1	117	92.79 ± 16.50	17.8	124
	400	429.3 ± 51.6	12.0	107	413.4 ± 52.2	12.6	103
	4000	3716 ± 311	8.36	92.9	4290 ± 700	16.3	107
6′-OH-CLZ	15	16.33 ± 4.71	28.8	109	16.64 ± 3.60	21.6	111
	80	84.17 ± 14.1	16.8	105	81.91 ± 34.78	12.8	116
	800	678.3 ± 157.8	23.3	84.8	840.1 ± 194.5	23.2	105