Intra- and interday precision and accuracy for LC-MS/MS quantification of P450 probe drugs and corresponding metabolites in mouse plasma
Concentrations are given in nanomoles. Values represent means ± S.D. or percentages unless otherwise indicated.
Analyte | Nominal Concentration | Intra-assay (n = 3) | Interassay (n = 9) | ||||
---|---|---|---|---|---|---|---|
Measured Concentration | Relevant S.D. | Accuracy | Measured Concentration | Relevant S.D. | Accuracy | ||
PHE | 75 | 69.93 ± 18.70 | 26.7 | 93.2 | 75.69 ± 10.84 | 14.3 | 101 |
400 | 387.7 ± 20.8 | 5.38 | 96.8 | 394.9 ± 15.4 | 3.89 | 98.6 | |
4000 | 3580 ± 114 | 3.17 | 89.4 | 3707 ± 168 | 4.54 | 92.6 | |
APAP | 15 | 14.97 ± 1.85 | 12.4 | 99.9 | 15.69 ± 1.23 | 7.84 | 104 |
80 | 81.57 ± 0.96 | 1.18 | 102 | 78.96 ± 2.38 | 3.02 | 98.7 | |
800 | 699.7 ± 11.8 | 1.69 | 87.4 | 722.6 ± 36.5 | 5.05 | 90.3 | |
MDZ | 15 | 15.40 ± 1.95 | 12.7 | 103 | 14.63 ± 1.98 | 13.6 | 97.5 |
80 | 73.77 ± 5.13 | 6.95 | 92.2 | 77.96 ± 9.29 | 11.9 | 97.4 | |
800 | 688.7 ± 66.3 | 9.62 | 86.1 | 721.3 ± 57.2 | 7.93 | 90.2 | |
1′-OH-MDZ | 75 | 71.47 ± 3.50 | 4.90 | 95.4 | 74.56 ± 6.18 | 8.29 | 99.4 |
400 | 382.3 ± 23.0 | 6.02 | 95.6 | 378.0 ± 14.4 | 3.80 | 94.5 | |
4000 | 3523 ± 136 | 3.86 | 88.1 | 3661 ± 272 | 7.42 | 91.6 | |
DXM | 75 | 64.67 ± 10.80 | 16.7 | 83.0 | 66.46 ± 7.47 | 11.2 | 87.5 |
80 | 356.0 ± 14.7 | 4.14 | 89.0 | 371.8 ± 19.6 | 5.27 | 92.9 | |
800 | 3767 ± 186 | 4.93 | 94.2 | 3741 ± 161 | 4.29 | 93.6 | |
DXO | 15 | 12.67 ± 2.63 | 20.7 | 84.6 | 12.87 ± 2.14 | 16.7 | 85.8 |
400 | 67.90 ± 4.48 | 6.60 | 84.9 | 72.92 ± 5.45 | 7.48 | 91.1 | |
4000 | 700.0 ± 25.9 | 3.71 | 87.5 | 736.8 ± 39.4 | 5.35 | 92.1 | |
DIC | 75 | 86.20 ± 6.77 | 7.86 | 115 | 90.33 ± 21.56 | 23.9 | 121 |
400 | 468.3 ± 11.2 | 2.40 | 117 | 421.8 ± 48.6 | 11.5 | 106 | |
4000 | 3647 ± 123 | 3.38 | 91.1 | 4148 ± 604 | 14.6 | 104 | |
4′-OH-DIC | 15 | 15.70 ± 2.48 | 15.8 | 105 | 17.61 ± 3.02 | 17.2 | 118 |
80 | 86.87 ± 15.76 | 18.2 | 109 | 81.39 ± 12.65 | 15.6 | 102 | |
800 | 826.3 ± 20.2 | 2.45 | 103 | 876.6 ± 102.8 | 11.7 | 110 | |
CLZ | 75 | 87.60 ± 8.88 | 10.1 | 117 | 92.79 ± 16.50 | 17.8 | 124 |
400 | 429.3 ± 51.6 | 12.0 | 107 | 413.4 ± 52.2 | 12.6 | 103 | |
4000 | 3716 ± 311 | 8.36 | 92.9 | 4290 ± 700 | 16.3 | 107 | |
6′-OH-CLZ | 15 | 16.33 ± 4.71 | 28.8 | 109 | 16.64 ± 3.60 | 21.6 | 111 |
80 | 84.17 ± 14.1 | 16.8 | 105 | 81.91 ± 34.78 | 12.8 | 116 | |
800 | 678.3 ± 157.8 | 23.3 | 84.8 | 840.1 ± 194.5 | 23.2 | 105 |