CYP content, G6Pase activity, and MPPG measured in homogenate and microsomal samples prepared from fresh dog kidney cortex, frozen dog kidney cortex, and frozen dog liver
Average values are presented, with CVs in parentheses. G6Pase activity was not measured in samples prepared from fresh dog kidney cortex. Data for individual dogs are presented (Supplemental Table S2).
| CYP Content (nmol/mg Protein) | G6Pase Activity (nmol/min/mg Protein) | MPPG (mg/g Tissue) | |||||
|---|---|---|---|---|---|---|---|
| Homogenate | Microsomes | Homogenate | Microsomes | CYP content | G6Pase activity | ||
| Fresh tissue (n = 14) | Dog kidney cortex | 0.086 (24%) | 0.205 (23%) | Not measured | Not measured | 43.1 (22%) | Not measured |
| Dog intestinea | Data not available | 0.059 (27%) | Not measured | Not measured | 6.5 (61%) | Not measured | |
| Frozen tissue (n = 17) | Dog kidney cortex | 0.056 (16%) | 0.230 (15%) | 19.9 (16%) | 62.1 (16%) | 33.9 (18%) | 44.0 (16%) |
| Dog liver | 0.113 (19%) | 0.665 (20%) | 23.8 (15%) | 91.2 (18%) | 41.1 (12%) | 63.6 (18%) | |
↵a Data for dog intestine were provided by Dr Oliver Hatley (manuscript in preparation) and represent data pooled from several intestinal regions.