Rank orders, abundance levels, and primary subcellular localization of the 10 most abundant proteins in the analyzed HLM samples and those of drug-metabolizing cytochrome P450 enzymes
| Overall Rank | Protein (Gene Name) | Overall Abundance | HLM01 | HLM02 | HLM03 | HLM04 | |
|---|---|---|---|---|---|---|---|
| Mean ± S.D.a [pmol mg−1] | Mean ± S.D.b [pmol mg−1] | Mean ± S.D.b [pmol mg−1] | Mean ± S.D.b [pmol mg−1] | Mean ± S.D. b [pmol mg−1] | |||
| (Rank) | (Rank) | (Rank) | (Rank) | ||||
| Top 10 HLM proteins | 1 | Liver carboxylesterase 1c | 403.14 ± 92.8 | 485.94 ± 24.31 (1) | 396.81 ± 31.63 (1) | 275.38 ± 13.45 (5) | 454.42 ± 19.15 (1) |
| 2 | Cytoplasmic actin 1 (ACTB)d | 316.94 ± 74.63 | 325.51 ± 50.06 (8) | 363.32 ± 21.94 (2) | 370.02 ± 24.37 (2) | 208.93 ± 23.54 (10) | |
| 3 | Protein disulfide isomerase (P4HB)c | 304.25 ± 101.48 | 415.74 ± 12.23 (3) | 324.86 ± 18.57 (3) | 169.96 ± 4.02 (9) | 306.44 ± 12.18 (2) | |
| 4 | 78-kDa glucose-regulated protein (HSPA5)c | 284.23 ± 62.67 | 352.90 ± 10.52 (6) | 284.86 ± 24.83 (5) | 201.29 ± 2.06 (8) | 297.86 ± 10.86 (3) | |
| 5 | ATP synthase subunit β (ATP5B)e | 264.10 ± 96.66 | 140.23 ± 1.59 (24) | 270.66 ± 25.62 (6) | 376.41 ± 19.64 (1) | 269.09 ± 2.31 (5) | |
| 6 | Protein disulfide isomerase A3 (PDIA3)c | 262.73 ± 108.03 | 387.29 ± 12.61 (4) | 252.14 ± 17.28 (7) | 127.46 ± 2.23 (27) | 286.05 ± 12.36 (4) | |
| 7 | Calreticulin (CALR)c | 257.40 ± 86.90 | 372.06 ± 7.27 (5) | 223.98 ± 36.44 (10) | 166.32 ± 13.55 (11) | 267.26 ± 4.77 (6) | |
| 8 | Haptoglobin (HP)f | 253.47 ± 155.76 | 457.21 ± 34.92 (2) | 292.00 ± 22.60 (4) | 117.54 ± 10.34 (30) | 147.11 ± 1.66 (21) | |
| 9 | Endoplasmin (HSP90B1)c | 243.64 ± 78.95 | 335.09 ± 9.03 (7) | 231.93 ± 17.82 (9) | 144.52 ± 15.96 (14) | 263.01 ± 6.13 (7) | |
| 10 | Cytochrome b5 (CYB5A)c | 226.79 ± 18.36 | 251.61 ± 26.34 (11) | 209.61 ± 15.40 (12) | 217.01 ± 7.03 (6) | 228.93 ± 36.13 (8) | |
| Drug-metabolizing P450 enzymesc | 44 | CYP3A4 | 80.87 ± 58.48 | 126.83 ± 4.43 (26) | 134.71 ± 13.65 (26) | 19.32 ± 1.63 (231) | 42.64 ± 1.02 (122) |
| 53 | CYP2E1 | 76.28 ± 14.78 | 73.50 ± 6.23 (57) | 74.36 ± 8.85 (65) | 60.84 ± 6.38 (78) | 96.43 ± 13.80 (34) | |
| 101 | CYP2C9 | 50.37 ± 30.63 | 94.26 ± 13.22 (43) | 29.89 ± 0.73 (192) | 28.82 ± 0.40 (171) | 48.49 ± 2.83 (106) | |
| 108 | CYP4F | 48.31 ± 19.96 | 28.37 ± 2.56 (165) | 68.91 ± 8.19 (70) | 34.32 ± 1.79 (142) | 61.63 ± 2.33 (80) | |
| 109 | CYP2A6 | 48.11 ± 43.44 | 108.60 ± 13.01 (36) | 38.44 ± 3.53 (147) | 5.11 ± 0.57 (597) | 40.30 ± 1.37 (127) | |
| 180 | CYP3A5 | 31.07 ± 9.68 | 37.92 ± 4.13 (117) | 24.22 ± 1.54 (231) | — | — | |
| 186 | CYP1A2 | 30.44 ± 7.19 | 24.25 ± 2.59 (184) | 28.72 ± 2.81 (196) | — | 38.33 ± 5.88 (134) | |
| 201 | CYP2C8 | 27.57 ± 25.42 | 64.37 ± 3.03 (66) | 23.22 ± 1.72 (244) | 6.92 ± 0.77 (516) | 15.78 ± 1.25 (296) | |
| 262 | CYP2B6 | 20.98 ± 1.22 | 20.98 ± 1.22 (225) | — | — | — | |
| 425 | CYP2D6 | 12.45 ± 4.84 | 10.74 ± 1.07 (372) | 8.70 ± 1.72 (538) | 17.91 ± 1.79 (245) | — | |
| 560 | CYP3A7 | 9.21 ± 0.62 | 9.21 ± 0.62 (413) | — | — | — | |
| 815 | CYP2C19 | 5.47 ± 0.69 | 5.96 ± 0.17 (542) | — | — | 4.98 ± 0.76 (604) | |
| 1066 | CYP3A43 | 1.06 ± 0.40 | 0.66 ± 0.11 (711) | 1.20 ± 0.12 (814) | 1.04 ± 0.11 (706) | 1.46 ± 0.05 (707) | |
| 51 | NADPH cytochrome P450 reductase (POR)c | 77.95 ± 19.18 | 85.88 ± 3.27 (53) | 100.12 ± 5.52 (35) | 55.97 ± 8.61 (87) | 69.85 ± 3.27 (62) |