Renal OATs mediated transport of glyburide and metabolites
Uptake by HEK cells stably transfected with human OAT1 and OAT3, normalized to uptake by wild-type HEK cells, are presented as uptake ratios. All data represent the mean ± SD (n = 3).
| Compound | OAT1 | OAT3 |
|---|---|---|
| Glyburide 1 µM | 1.24 ± 0.16 | 1.25 ± 0.10 |
| Glyburide 10 µM | 1.25 ± 0.09 | 0.97 ± 0.07 |
| M1 1 µM | 1.48 ± 0.20 | 6.22 ± 0.04 |
| M1 10 µM | 0.37 ± 0.02 | 11.50 ± 0.86 |
| M2b 1 µM | 0.64 ± 0.06 | 9.25 ± 0.54 |
| M2b 10 µM | 0.96 ± 0.63 | 13.51 ± 0.72 |
| 3H-para-aminohippuric acid (hOAT1)a | 74.13 ± 5.69 | |
| 3H-estrone sulfate (hOAT3)a | 95.04 ± 1.98 |
hOAT, human OAT.
↵a Probe substrate used as positive control for the respective transporter.