TABLE 1

General characterization of mAOX1, mAOX3, mAOX4, and mAOX2 after expression in E. coli TP1000 cells

Sample	Specific Activity^{^a}		Yield	Ratio (280/450)	Ratio (450/550)
Sample	As Purified	After Chemical Sulfuration	Yield	Ratio (280/450)	Ratio (450/550)
	nmol min⁻¹ mg⁻¹	nmol min⁻¹ mg⁻¹	mg/l of E. coli Culture
mAOX1	N.D.	146.7 ± 19.5	1.2 ± 0.2	5.7	3.2
mAOX3^{^b}	104^{^b}	158^{^b}	0.8^{^b}	5.2^{^b}	3.1^{^b}
mAOX4	48.4 ± 8.1	585.2 ± 37.3	1.1 ± 0.4	5.4	3.1
mAOX2	N.D.	880.4 ± 27.7	0.8 ± 0.2	5.0	3.2

N.D., no activity was detectable.
↵^a Specific activity was measured using 50 μM salicylaldehyde as substrate and 100 μM DCPIP as electron acceptor.
↵^b Data published by Mahro et al. (2011) with benzaldehyde as substrate.