TABLE 1 

Method performance: lower limit of quantification (LLOQ), calibration range, between-assay precision (RSD), and accuracy (bias)

AnalyteLLOQ Calibration RangeQC LowQC MediumQC High
MeanRSDBiasMeanRSDBiasMeanRSDBias
 µM µM
PMMA0.0251–1502.111%3%2320%13%10010%−1%
PMA0.010.01–30.02823%−6%0.7125%−12%7.120%−12%
MDMA0.051–1504.612%13%4415%9%1119%11%
MDA0.0250.025–7.50.06919%−6%1.810%−10%1822%−10%
Methamphetamine0.051–1504.110%3%3912%−2%1018%1%
Amphetamine0.050.05–150.07019%−4%1.86%−9%187%−8%
OH-MA0.10.1–1000.1318%−9%3.613%−9%3717%−8%
OH-A0.010.01–1.70.01331%−15%0.4114%0%3.517%−15%
Di-OH-MA0.010.01–30.03020%2%0.7913%−1%6.614%−17%
Oxilofrine0.010.01–1.50.01517%−3%0.4112%−2%2.616%−36%
HM-MA0.010.01–1.50.01519%−3%0.4022%0%3.613%−11%
HM-A0.0050.005–0.760.007719%10%0.208%2%2.014%−2%
  • di-OH-MA, 3,4-dihydroxymethamphetamine; HM-A, 4-hydroxy-3-methoxyamphetamine; HM-MA, 4-hydroxy-3-methoxymethamphetamine; OH-A, 4-hydroxyamphetamine; OH-MA, 4-hydroxymethamphetamine.