TABLE 1 

Rates of LA and AA metabolite formation by CYP4Z1 microsomes

SubstrateaMetaboliteRate (pmol/pmol per minute)
LA7-OH LA∼2b
LA8-OH LA∼40b
LA9-OH LA6.1 ± 0.7
LA10-OH LA16.0 ± 1.4
LA11-OH LA8.8 ± 0.2
LA12-OH LAn.d.
AA19-HETE0.048 ± 0.004
AA20-HETEn.d.
AA20-COOH-AAn.d.
AA14,15-EET1.9 ± 0.06

  • n.d., not detected.

  • ↵a Substrate concentrations: 1 mM LA; 75 μM AA.

  • ↵b Since we lacked synthetic standards for the 7- and 8-hydroxy metabolites of LA, these values were estimated using the 9-OH LA calibration curve.