Protein expression of oatps and transport-mediated intrinsic of RSV in CHO-Oatp1a1, HEK293-Oatp1a4, and HEK293-Oatp1b2 cells
Data are the mean ± S.D. of triplicate determination. Transporter-mediated uptake CL of RSV was calculated as the ratio of RSV transporter-mediated uptake (uptake in transfected cells minus that in mock cells) over 5 s (Oatp1a1) or 15 s (Oatp1a4 and Oatp1b2) and the total buffer RSV concentration (0.07 µM).
Oatp Isoform | Transporter Expression | Intrinsic ![]() | |
---|---|---|---|
µl/min per mg total protein | µl/min per femtomol transporter | ||
fmol/µg membrane protein | |||
1a1 | 6.72 ± 0.63 | 459 | 0.113 |
1a4 | 2.61 ± 0.25 | 54.0 | 0.043 |
1b2 | 18.1 ± 1.79 | 143 | 0.015 |