TABLE 6 

Protein binding of MMB, M19, and M21 in plasma, hepatic microsomal fraction, and cell culture media

MatrixFraction Unbound, % (mean ± S.D.)
MMBM19M21
PlasmaSprague Dawley rat2.5 ± 0.23.6 ± 0.10.9 ± 0.1
Beagle dog19.2 ± 0.725.3 ± 0.232.5 ± 3.7
Human19.2 ± 0.512.2 ± 0.214.6 ± 0.6
Hepatic microsomal fractionHuman92.781.596.3
Cell culture medium1% FCS in EMEM80.1NA84.6
10% FCS63.4NA66.5
  • EMEM, Eagle’s minimum essential medium; FCS, fetal calf serum; NA, not applicable.