TABLE 2

Extraction recovery of the radioactivity from in vitro incubations of [14C]MLN3126 at a final concentration of 5 μg/ml in blank plasma and protein solutions for 16 h at 37°C

NaBH4 (+) TFA(+) indicates extracted with 5 volumes of acetonitrile after treatment with NaBH4 followed by treatment with TFA; TFA (−) indicates extracted with 5 volumes of methanol; and TFA (+) indicates extracted with 5 volumes of acetonitrile after treatment with TFA. Mean values: n = 3.

SampleExtraction Recovery (%)
TFA (−)TFA (+)NaBH4 (+) TFA (+)
Rat plasma54.9104.9N.D.
Human plasma47.3104.7N.D.
Dog plasma74.8104.6N.D.
1% RSA30.2103.831.9
1% HSA41.0103.039.1
1% DSA81.7105.078.0
1% AGP105.7103.6N.D.
1% Globulins100.4100.8N.D.
  • N.D., not determined.