Medians and 95% confidence intervals of estimated parameters in an SCHH model and a PMH model and published parameter values in a PRH model
| Parameter | SCHH | PMH | PRH |
|---|---|---|---|
| CLHEP,pass (μl/min per mg) | 10.8 (9.7, 12) | 3.55 (2.2, 5.3) | 13.5 |
| kHEP,uptake (pmol/min per mg) | 96.3 (67, 140) | 24.6 (15, 39) | 488 |
| KM,HEP,uptake (nM) | 4340 (2800, 6800) | 1280 (800, 2000) | 6400 |
| kHEP,efflux (pmol/min per mg) | 0.117 (0.084, 0.15) | 68.5 (25, 170) | |
| KM,HEP,efflux (nM) | 0.0100 | 7100 (2400, 2.1 × 104) | |
| kHEP,metabolism (pmol/min per mg) | 3.08 × 10−8 | 5.90 × 10−9 | |
| KM,HEP,metabolism (nM) | 6.9 × 104 (fixed) | 2.85 × 104 (fixed) | |
| kon,HEP,IC (1/nM/min) | 60 (fixed) | 60 (fixed) | |
| koff,HEP,IC (min−1) | 8.45 × 105 (5.8 × 105, 1.2 × 106) | 8.45 × 105 (fixed) | |
| Total intracellular binding site (nM) | 3.82 × 105 (3.1 × 105, 4.7 × 105) | 3.82 × 105 (fixed) | |
| Ka,EC (ml/mg) | 2.75 × 10−3 (1.8 × 10−3, 3.7 × 10−3) | 0.0163 (0.0133, 0.0194) | |
| Cell number (million cells/mg measured protein) | 2.5 (fixed) | 2.5 (fixed) | |
| Cell volume (ml/million cells) | 2.6 × 10−3 (fixed) | 2.6 × 10−3 (fixed) | |
| VHEP,EC (ml/well) | 0.5 (fixed) | 0.5 (fixed) |
PRH data are published in a previous study (Ménochet et al., 2012). Medians and confidence intervals are not provided for the parameters with high uncertainty (i.e., the range of the approximated confidence interval is greater than 20 magnitude); the globally optimized value is provided instead.