Cisplatin-induced toxicity is independent of OAT-mediated transport in ciPTEC-OAT1 and ciPTEC-OAT3
TC50 values (represented in micromolar) calculated from MTT viability assays following cisplatin (8.7–222 μM) exposure for 24 h are not affected by 24 h (n = 3) and 7-day (n = 6) competitive inhibition of OAT (probenecid, 100 μM; diclofenac, 50 μM) in ciPTEC-parent, ciPTEC-OAT1, and ciPTEC-OAT3 (mean TC50 ± S.E.M., *P < 0.05; ***P < 0.001; compared with ciPTEC-parent, no significant differences were found for treatments by two-way analysis of variance, N.D. is not determined).
| Coexposure (24 Hours) | Co- and Pre-exposure (7 Days) | ||||
|---|---|---|---|---|---|
| – | + | – | + | ||
| ciPTEC-parent | Probenecid | 33 ± 3 | 29 ± 4 | 47 ± 4 | 45 ± 3 |
| Diclofenac | 34 ± 1 | 34 ± 1 | N.D. | N.D. | |
| ciPTEC-OAT1 | Probenecid | 53 ± 2* | 49 ± 3 | 45 ± 6 | 45 ± 6 |
| Diclofenac | 45 ± 6 | 45 ± 4 | N.D. | N.D. | |
| ciPTEC-OAT3 | Probenecid | 92 ± 8*** | 93 ± 7*** | 80 ± 2*** | 76 ± 6*** |
| Diclofenac | 64 ± 11* | 62 ± 13* | N.D. | N.D. | |