TABLE 1

Comparison of the average protein abundance and range (interindividual variability) in protein abundance for four human liver tissue samples measured with two different proteomic methodologies, targeted proteomics, and multiplexed global proteomics using tandem mass tag (TMT) reagents

LC-MS/MS/MS run time was 24 hours for laboratory 1 and 48 hours for laboratory 2.

TransporterAverage Protein Abundance ± S.D. 
(pmol/mg membrane protein)Range in Protein Abundance 
(max/min)
Targeted DataTMT DataTargeted DataTMT Data
Laboratory 1Laboratory 2Laboratory 1Laboratory 2
NTCP1.28 ± 0.671.09 ± 0.39a4.81 ± 1.52*6.032.60a2.32
OAT21.15 ± 0.450.74 ± 0.322.74 ± 0.94*2.502.712.30
OAT75.42 ± 1.771.32 ± 0.55*4.16 ± 1.38*1.972.531.97
OATP1B18.08 ± 2.242.80 ± 0.85*4.28 ± 1.47*2.072.242.52
OATP1B34.39 ± 1.881.24 ± 0.24*2.11 ± 0.98*2.601.582.69
OATP2B11.86 ± 0.731.72 ± 0.502.29 ± 0.792.231.962.35
OCT12.92 ± 0.805.69 ± 2.31*3.37 ± 1.001.792.321.86
  • * Statistically different from the targeted proteomic data (adjusted P < 0.05).

  • a NTCP values represent MS2 data for laboratory 1 because of lack of detection of NTCP in MS3 mode.