TABLE 1

First-step screening for the evaluation of transporter expression in Caucasian skin samples

In easy comparisons (first screening), the mean cycle threshold (C_t) value of each transporter was calculated. One-sixteenth of pooled cDNA was used as a template for real-time PCR. Pooled cDNA was prepared by mixing an identical volume of cDNA (n = 20). C_t values >35, in the range of 31–35, and <31 were interpreted as the absence of gene expression, the presence of gene expression but below the limit of quantitation, and the presence of gene expression at the quantitative level, respectively.

Gene	C_t Mean	Gene	C_t Mean	Gene	C_t Mean	Gene	C_t Mean
ABCA1	28.9	ABCC2	33.4	SLC15A1	30.6	SLC22A8	>35
ABCA2	29.1	ABCC3	27.0	SLC15A2	32.5	SLC22A10	>35
ABCA3	31.1	ABCC4	32.2	SLC16A1	29.7	SLC22A11	>35
ABCA4	>35	ABCC5	28.4	SLC16A3	33.3	SLC46A1	32.5
ABCA5	29.0	ABCC6	32.2	SLC16A7	30.0	SLC47A1	29.2
ABCA6	32.3	ABCC8	>35	SLC16A8	>35	SLC47A2	30.4
ABCA7	30.7	ABCC10	33.2	SLC22A1	32.9	SLCO1A2	>35
ABCA8	31.2	ABCC11	33.6	SLC22A2	>35	SLCO1B1	>35
ABCA9	32.1	ABCC12	>35	SLC22A3	28.6	SLCO1B3	>35
ABCA10	31.2	ABCG1	29.9	SLC22A4	33.0	SLCO2B1	30.1
ABCA12	31.8	ABCG2	32.9	SLC22A5	30.2	SLCO3A1	28.9
ABCB1	32.1	ABCG5	34.4	SLC22A6	>35	SLCO4A1	33.7
ABCC1	29.3	ABCG8	>35	SLC22A7	>35