TABLE 4

Cyclosporine toxicity in ciPTEC in 2D cultures and recellularized scaffolds in this study in comparison with published in vitro data

ConditionTC50 (μM)Reference
PrestoBlue assayciPTEC–OAT1 2D129 ± 12
ciPTEC–OAT1 2D (+Zsq 1 μM)35 ± 6
RS294 ± 13
RS (+Zsq 1 μM)87 ± 10
Live/dead assayRS15.0 ± 1.2
RS (+Zsq 1 μM)1.6 ± 0.4
LiteratureHK-2 (72 h)>50Huang et al. (2015)
hPTECs (72 h)>50
HRECs (CsA only; 144 h)7.4 ± 0.5Anglicheau et al. (2006)
hRECs (CsA + PSC833 5 μM; 144 h)3.7 ± 0.5
  • HK-2, human kidney cells 2; hPTECs, human proximal tubule epithelial cells; hRECs, human renal epithelial cells.