Cyclosporine toxicity in ciPTEC in 2D cultures and recellularized scaffolds in this study in comparison with published in vitro data
| Condition | TC50 (μM) | Reference | |
|---|---|---|---|
| PrestoBlue assay | ciPTEC–OAT1 2D | 129 ± 12 | |
| ciPTEC–OAT1 2D (+Zsq 1 μM) | 35 ± 6 | ||
| RS | 294 ± 13 | ||
| RS (+Zsq 1 μM) | 87 ± 10 | ||
| Live/dead assay | RS | 15.0 ± 1.2 | |
| RS (+Zsq 1 μM) | 1.6 ± 0.4 | ||
| Literature | HK-2 (72 h) | >50 | Huang et al. (2015) |
| hPTECs (72 h) | >50 | ||
| HRECs (CsA only; 144 h) | 7.4 ± 0.5 | Anglicheau et al. (2006) | |
| hRECs (CsA + PSC833 5 μM; 144 h) | 3.7 ± 0.5 |
HK-2, human kidney cells 2; hPTECs, human proximal tubule epithelial cells; hRECs, human renal epithelial cells.