TABLE 1

Gene expression levels, in cycle threshold (Ct) of ciPTEC–OAT1 in 2D cultures, in recellularized scaffolds cultures (3D), and in human kidney (HuK)

Data are shown as mean ± S.D. of three independent experiments performed, at least, in duplicate.

Ct (2D)Ct (3D)Ct (HuKa)
Housekeeping genesGAPDH22.4 ± 1.222.7 ± 0.425.5 ± 1.1
HPRT123.1 ± 0.123.5 ± 0.4n.a.
Epithelial markersZO-124.0 ± 0.328.2 ± 0.9n.a.
CLDN-232.7 ± 0.830.7 ± 0.8n.a.
AQP-130.6 ± 0.628.5 ± 1.2n.a.
Physiologically relevant markersOCT-233.9 ± 4.229.6 ± 1.430.3 ± 1.8
OAT-126.2 ± 1.125.0 ± 5.124.6 ± 1.6
P-gp30.8 ± 1.128.8 ± 4.530.3 ± 1.8
BCRP30.6 ± 1.629.7 ± 1.6n.a.
MRP-429.4 ± 1.128.8 ± 3.729.3 ± 1.0
MATE-130.6 ± 0.425.2 ± 1.2n.a.
MATE-2K28.8 ± 0.526.7 ± 1.0n.a.
33.9 ± 4.229.6 ± 1.430.3 ± 1.8
GLUT-126.2 ± 1.125.0 ± 5.124.6 ± 1.6
  • OATP4C1, organic anion transporter polypeptide 4C1.

  • a HuK, human kidney, unpublished data provided by Vriend, J. et al.; n.a. (not available).