TABLE 3

Tenofovir toxicity in ciPTEC in 2D cultures and recellularized scaffolds in this study in comparison with published in vitro data

ConditionTC50 (μM)Reference
PrestoBlue assayciPTEC–OAT1 2D (24 h)212 ± 11
ciPTEC–OAT1 2D (48 h)85 ± 9
RS (24 h)97 ± 8
RS (48 h)93 ± 5
Live/Dead assayRS (24 h)11.0 ± 3.0
RS (48 h)5.5 ± 2.0
LiteratureciPTEC–OAT1 2D (24 h)114 ± 25Nieskens et al., 2016
ciPTEC–OAT1 2D (48 h)189 ± 48
ciPTEC–OAT1 2D (72 h)223 ± 67
CHO-hOAT1 (24 h)21 ± 7Cihlar et al. (2009)
HEK-293–OAT1 (24 h)10 ± 2Zhang et al. (2015)
  • HEK–OAT1, human embryonic kidney cells 293 overexpressing OAT-1.