Gene | Forward (5′ to 3′) | Reverse (5′ to 3′) |
---|---|---|
Genotyping | ||
Bmal1 | GCCCATGACCTCAGTGCCACA | CCGGCTTTAAGGCTATTGATTT |
RT-qPCR | ||
Sult1a1 | CACAAGGGTCCTCTCCTTAGC | TGACAGCGGAACGTGAAGTC |
Bmal1 | CTCCAGGAGGCAAGAAGATTC | ATAGTCCAGTGGAAGGAATG |
Dbp | ACATCTAGGGACACACCCAGTC | AAGTCTCATGGCCTGGAATG |
Ppib | TCCACACCCTTTTCCGGTCC | CAAAAGGAAGACGACGGAGC |
Plasmid construction | ||
Bmal1 | GATCTCGAGCCACCATGGCGGACCAGAGAATGGAC | GATGGATCCCTACAGCGGCCATGGCAAGTCAC |
Promoter construction | ||
Sult1a1 (−2018/+39) | GCCTCGAGGATATCAAGATCTGCCAAAATGGATTCCTCAG | CAGTACCGGATTGCCAAGCTTGCCTCCCTCGGTGC |
Sult1a1 (−660/+39) | CCGGTACCTGAGCTCGCTAGCCAGCCTCTTGTCTTTG | CCGGTACCTGAGCTCGCTAGCCAGCCTCTTGTCTTTG |
Sult1a1 (−400/+39) | GCCTCGAGGATATCAAGATCTCCAGGCATCGCTG | CAGTACCGGATTGCCAAGCTTGCCTCCCTCGGT |
EMSA | ||
Sult1a1 | TCTCCACCAGTCACGTGTGGTATTGGA | TCCAATACCACACGTGACTGGTGGAGA |
Sult1a1 (mutant) | TCTGCACCCGTATCACGTTGAATTGGA | TCCAATTCAACGTGATACGGGTGCAGA |
CHIP | ||
Dbp | TGGGACGCCTGGGTACAC | GGGAATGTGCAGCACTGGTT |
Sult1a1 | TGTCTTTGCAGAGGGTGA | GAATGATGGGAAGGAGCA |
Sult1a1(distal) | CTTTTAAGTATGCACAGG | AGAACTGACTGCTACATG |
Ppib, peptidyl-prolyl cis-trans isomerase B.