Quantitative targeted absolute proteomics of BBB fraction from Oatp1a4-humanized mice
Transporter protein expression in the brain capillary was quantified as described in Materials and Methods. The protein expression level of target molecule was determined as an average and variability (mean ± S.E.) of the quantitative values obtained from different SRM/multiple reaction monitoring (MRM) transitions in three analyses.
| Molecular names | Peptide Sequence | Protein Expression Level | |
|---|---|---|---|
| Control | Humanized | ||
| fmol/μg protein | |||
| OATP1A2^IY | IYDSTTFR | U.L.Q. (<0.076) | 0.45 ± 0.04 |
| OATP1A2^EG | EGLETNADIIK | U.L.Q. (<0.39) | 1.5 ± 0.36 |
| Oatp1a4 | EVATHGVR | 1.5 ± 0.24 | U.L.Q. (<0.062) |
| Mdr1a | NTTGALTTR | 13 ± 1.2 | 18 ± 0.5 |
| Oatp1c1 | STVTQIER | 1.0 ± 0.12 | 1.1 ± 0.11 |
| Oat3 | YGLSDLFR | 1.7 ± 0.04 | 1.7 ± 0.25 |
| Bcrp^SS | SSLLDVLAAR | 3.6 ± 0.04 | 4.3 ± 0.07 |
| Bcrp^EN | ENLQFSAALR | 3.0 ± 0.09 | 3.6 ± 0.12 |
| Mrp4 | APVLFFDR | 1.0 ± 0.14 | 1.3 ± 0.17 |
| Glut1 | TFDEIASGFR | 90 ± 2.3 | 100 ± 5.2 |
| Taut | EGATPFHSR | U.L.Q. (<1.5) | U.L.Q. (<1.0) |
| Tfr1 | SSVGTGLLLK | 3.1 ± 0.16 | 4.0 ± 0.20 |
| γ-gtp | LFQPSIQLAR | 3.2 ± 0.08 | 4.0 ± 0.29 |
| Na+/K+ ATPase | AAVPDAVGK | 48 ± 3.8 | 56 ± 2.2 |
Control, BBB fraction from the control mice; Humanized, that from the humanized mice; U.L.Q., under the limit of quantification.