TABLE 6

Distribution of P450 isoform activities in CHIM using midazolam 1′-hydroxylation as CYP3A4 activity

Results are expressed as the percentage of the arithmetic sum of the specific activities (in picomoles per minute per milligram protein) of the P450 isoforms evaluated.

IsoformTotal Activities
Donor 1Donor 2Donor 3Donor 4
DuodenumJejunumIleumDuodenumJejunumIleumCombinedDuodenumJejunumIleum
CYP1A14.672.657.264.345.7521.2217.6210.851.381.55
CYP1A216.3310.7719.1035.0222.8516.4023.5934.7544.1231.32
CYP2A60.030.010.020.050.080.590.000.000.000.00
CYP2B63.112.414.093.452.726.4110.9210.077.476.15
CYP2C80.270.280.350.220.220.480.761.360.931.94
CYP2C911.2429.3733.167.3312.014.352.404.322.834.66
CYP2C1915.347.323.1735.2511.020.001.523.437.049.02
CYP2D60.580.350.000.770.350.000.000.290.000.43
CYP2E11.160.811.460.083.6711.872.580.000.000.53
CYP3A4/5-136.0940.1718.666.8725.950.005.606.2113.9816.72
CYP2J211.185.8512.726.6115.3938.7035.0128.7222.2327.68
Sum of all isoforms100.00100.00100.00100.00100.00100.00100.00100.00100.00100.00