Overview of ADME and ADME-related genes edited using CRISPR-Cas9
| Gene/Protein | Cell Line or Organism | Modification | Reference |
|---|---|---|---|
| Family: Cytochrome P450s | |||
| CYP1A1/CYP1A1 | Induced pluripotent stem cells | Knockin of a Luciferase reporter cassette at the transcription start site | Smith et al. (2016) |
| Cyp2b9/10/13/CYP2B9/10/13 | C57BL/6 mice | Knockout of Cyp2b9, Cyp2b10 and Cyp2b13 | Kumar et al. (2017) |
| Cyp2c11/CYP2C11 | Sprague-Dawley rats | Knockout of Cyp2c11 | Wei et al. (2018) |
| Cyp2d1/2/3/4/5/CYP2D1/2/3/4/5 | Wistar rats | Knockout of the Cyp2d cluster | Yoshimi et al. (2016) |
| Cyp2d1/2/3/4/5/CYP2D1/2/3/4/5 | Wistar rats | Knockout of the rat Cyp2d cluster and knockin of human CYP2D6 | Yoshimi et al. (2016) |
| Cyp2e1/CYP2E1 | Sprague-Dawley rats | Knockout of Cyp2e1 | Wang et al. (2016) |
| cyp26a1/CYP26A1 | Silurus meridionalis | Knockout of cyp26a1 | Li et al. (2016) |
| Cyp3a1/2/CYP3A1/2 | Sprague-Dawley rats | Knockout of Cyp3a1 and Cyp3a2 | Lu et al. (2017) |
| CYP3A5/CYP3A5 | Human hepatocyte cell line HuH-7 | Editing of the CYP3A5*3 locus to generate CYP3A5*1 | Dorr et al. (2017) |
| CYP90B1/CYP90B1 (PtoDWF4) | Populus tomentosa | Knockout of CYP90B1 | Shen et al. (2018) |
| CYP734A4/CYP734A4 | Japonica rice cultivar Zhonghua11 | Knockout of CYP734A4 | Qian et al. (2017) |
| Family: ABC transporters | |||
| ABCA1/ABCA1 | Human pluripotent stem cells | Knockout of ABCA1 | Gupta et al. (2016) |
| ABCA2/ABCA2 | Helicoverpa armigera | Knockout of ABCA2 | Wang et al. (2017) |
| ABCB1/MDR1, ABCB1 or P-glycoprotein | Canine kidney cell line MDCK | Knockout of ABCB1 | Simoff et al. (2016) |
| ABCB1/MDR1, ABCB1 or P-glycoprotein | Human osteosarcoma cell lines KHOSR2 and U-2OSR2 | Knockout of ABCB1 | Liu et al. (2016) |
| ABCB1/MDR1, ABCB1 or P-glycoprotein | Human breast cancer cell line MCF-7/ADR | Knockout of ABCB1 | Ha et al. (2016) |
| ABCB1/MDR1, ABCB1 or P-glycoprotein | Human oral squamous carcinoma cell line KBv200 and human colorectal cancer cell line HCT-8/V | Knockout of ABCB1 | Yang et al. (2016b) |
| ABCB1/MDR1, ABCB1 or P-glycoprotein | Canine kidney cell line MDCK overexpressing human MDR1 | Knockout of ABCB1 | Karlgren et al. (2017) |
| ABCB1/MDR1, ABCB1 or P-glycoprotein | Human ovarian cancer cell line A2780/ADR | Knockout of ABCB1 | Norouzi-Barough et al. (2018) |
| ABCB1 ortholog MDR1/MDR1 | Spodoptera exigua | Knockout of MDR1 | Zuo et al. (2018) |
| ABCB1 ortholog mdr49/MDR49 | Drosophila melanogaster | Knockout of mdr49 | Denecke et al. (2017) |
| ABCB1 ortholog mdr50/MDR50 | Drosophila melanogaster | Knockout of mdr50 | Denecke et al. (2017) |
| ABCB1 ortholog mdr65/MDR65 | Drosophila melanogaster | Knockout of mdr65 | Denecke et al. (2017) |
| ABCB1 ortholog mdr1/mdr1 | Plasmodium falciparum | Introduction of point mutations in mdr1 | Ng et al. (2016) |
| ABCB1/MDR1 | Human acute lymphoblastic leukemia cell line HAL01 | Knockout of ABCB1 | Takahashi et al. (2017) |
| ABCB1/MDR1 | Human fibrosarcoma derived cells line HT-1080 | Edition of two GpG islands in the ABCB1 promoter | Grob et al. (2018) |
| ABCB11 ortholog abcb11b/ABCB11B | Zebrafish | Knockout of abcb11b | Ellis et al. (2018) |
| ABCB1 ortholog mrp1/MRP1 | Zebrafish | Knockout of mrp1 | Tian et al. (2017) |
| ABCC3/MRP3 | Human keratinocyte cell line HaCaT | Deletion of a CpG island 10 kbp upstream of ABCC3 | Takechi et al. (2018) |
| ABCC7, also known as CFTR/CFTR or ABC35 | Human colon carcinoma cell line T84 | Knockout of CFTR | Chung et al. (2016) |
| ABCC7, also known as CFTR/CFTR or ABC35 | Human tracheal epithelial cells from a cystic fibrosis patient (CFTEs) | Analysis of NHEJ and template dependent editing of the F508del mutation | Hollywood et al. (2016) |
| ABCC7, also known as CFTR/CFTR or ABC35 | Human adult intestinal cell lines | Correction of a 3 basepair deletion in CFTR | Schwank et al. (2013) |
| ABCC7, also known as CFTR/CFTR or ABC35 | Human lung adenocarcinoma cell line Calu-3 cells and primary human airway epithelial cells | Knockout of CFTR | Bellec et al. (2015) |
| ABCC7, also known as CFTR/CFTR or ABC35 | Human pluripotent stem cells | Correction of a three base pair deletion in CFTR | Firth et al. (2015) |
| ABCC7, also known as CFTR/CFTR or ABC35 | Human colon cancer cell line Caco-2 | Deletion of a 1.2 kb fragment/a CTCF binding site located 20.9 kb upstream of the transcription start site | Yang et al. (2016a) |
| ABCC7, also known as CFTR/CFTR or ABC35 | Human colon cancer cell line Caco-2 | Deletion of a 1.2 kb fragment/a binding site for intestinal selective enhancers located in intron 11 | Yang et al. (2016a) |
| ABCC7, also known as CFTR/CFTR or ABC35 | Human CFTE cell line | Editing of three rare mutations in CFTR | Sanz et al. (2017) |
| ABCC7, also known as CFTR/CFTR or ABC35 | Human bronchial epithelial cell line 16HBE | Introduction of a CFTR mutation | Molinski et al. (2017) |
| ABCC7, also known as CFTR/CFTR or ABC35 | Conditionally reprogramed human bronchial epithelial cells | Knockout of CFTR | Peters-Hall et al. (2018) |
| ABCC8/ABCC8 | Human embryonic stem cells | Knockout of ABCC8 | Guo et al. (2016a,b, 2017) |
| ABCG family member Bm-brown/Bm-brown | Bombyx mori | Knockout of Bm-brown | Zhang et al. (2018) |
| ABCG family member white (w) | Drosophila subobscura | Mutating the white gene | Tanaka et al. (2016) |
| ABCG family member white (w) | Drosophila melanogaster | Mutating the white gene | Kondo and Ueda (2013) |
| ABCG family members white (Ha-w), scarlet (Ha-st), brown (Ha-bw) and ok (Ha-ok) | Helicoverpa armigera | Knockout of white, scarlet, brown and ok | Khan et al. (2017) |
| Family: SLC transporters | |||
| SLC31A1/CTR1 | Human embryonic kidney cell line HEK293T and human ovarian cell line OVCAR8 | Knockout of SLC31A1 | Tsai et al. (2015), Bompiani et al. (2016) |
| SLC31A2/CTR2 | Human embryonic kidney cell line HEK293T and human ovarian cell line OVCAR8 | Knockout of SLC31A2 | Tsai et al. (2015), Bompiani et al. (2016) |
| SLC35B2/SLC35B2 or UGTrel4 | Primary human CD4+ T cells | Knockout of SLC35B2 | Park et al. (2017) |
| SLC35F2/SLC35F2 | Papillary thyroid carcinoma cell lines BCPAP and KTC-1 | Knockout of SLC35F2 | He et al. (2018) |
| slc45a2/SLC45A2 | Atlantic salmon | Indel formation in slc45a2 | Edvardsen et al. (2014) |
| Slco2a1/OATP2A1 | Mouse mammary cell line C127 | Knockout of Slco2a1 | Sabirov et al. (2017) |
| Family: Others | |||
| AHR2A/B/aryl hydrocarbon receptor 2A/B (AHR2A/B) | Fundulus heteroclitus | Insertion of mutations in AHR2A and AHR2B | Aluru et al. (2015) |
| AhR/aryl hydrocarbon receptor (AhR) | Human embryonic stem cells | Knockout of AhR | Angelos et al. (2017) |
| Ahr/aryl hydrocarbon receptor (AhR) | Young adult mouse colonic cells | Knockout of Ahr | Cheng et al. (2017) |
| AhR/aryl hydrocarbon receptor (AhR) | Human Mammary carcinosarcoma cell line Hs578T | Knockout of AhR | Narasimhan et al. (2018) |
| ahr2/aryl hydrocarbon receptor 2 (AhR2) | zebrafish | Knockout of ahr2 | Garcia et al. (2018) |
| POR/cytochrome P450 oxidoreductase (POR or CYPOR) | Human embryonic kidney cell line HEK293 | Knockout of POR | Rezende et al. (2017) |
| PXR or NR1I2/pregnane X receptor (PXR) or NR1I2 | Induced pluripotent stem cells | Knockin of an mCherry tag | Kim et al. (2018) |
| VKORC1/vitamin K epoxide reductase complex subunit 1 and VKORC1L1/vitamin K epoxide reductase complex subunit 1-like protein 1 | Human embryonic kidney cell line HEK293T | Knockout of VKORC1 and VKORC1L1 | Czogalla et al. (2017) |
ABC, ATP-binding cassette; AhR, aryl hydrocarbon receptor; CFTE, cystic fibrosis tracheal epithelial; SLC, solute carrier.