TABLE 7

Distribution of P450 isoform activities in CHIM using testosterone 6β-hydroxylation as CYP3A4 activity

Results are expressed as the percentage of the arithmetic sum of the specific activities (in picomoles per minute per milligram protein) of the P450 isoforms evaluated.

IsoformTotal Activities
Donor 1Donor 2Donor 3Donor 4
DuodenumJejunumIleumDuodenumJejunumIleumCombinedDuodenumJejunumIleum
CYP1A10.870.552.401.051.8015.048.883.940.500.43
CYP1A23.062.236.328.507.1311.6211.9012.6216.078.69
CYP2A60.010.000.010.010.020.410.000.000.000.00
CYP2B60.580.501.350.840.854.555.503.662.721.71
CYP2C80.050.060.120.050.070.340.380.500.340.54
CYP2C92.106.0810.971.783.753.081.211.571.031.29
CYP2C192.871.521.058.553.440.000.771.242.572.50
CYP2D60.110.070.000.190.110.000.000.110.000.12
CYP2E10.220.170.480.021.158.411.300.000.000.15
CYP3A4/5-288.0387.6173.0877.4076.8929.1252.4065.9268.6676.90
CYP2J22.091.214.211.604.8027.4317.6510.438.107.68
Sum of all isoforms100.00100.00100.00100.00100.00100.00100.00100.00100.00100.00