AOX-1 protein content in human liver cytosol, microsomes, and S9 fraction, as quantified in a capillary nano-proteomic immunoassay (Simple Western system; ProteinSimple)
Data are expressed as mean ± S.D. of three independent experiments.
| Sample | AOX-1 Protein Content | |
|---|---|---|
| pmol/mg of total proteina | percentage relative to cytosol | |
| Human liver cytosol | 63.8 ± 7.8 | 100% |
| Human liver microsomes, Pool 1 | 12.3 ± 1.4 | 19% |
| Human liver microsomes, Pool 2 | 13.0 ± 1.4 | 20% |
| Human liver microsomes, Pool 3 | 23.1 ± 1.5 | 36% |
| Human liver microsomes, Pool 4 | 15.1 ± 1.1 | 24% |
| Human liver microsomes, Pool 5 | 16.1 ± 1.2 | 25% |
| Human liver S9 fraction | 53.3 ± 3.5 | 83% |
↵a Micrograms per milligram of total protein was converted to picomoles per milligram of total protein, on the basis of molecular weight of 147,918 Da for AOX-1 (UniProt Knowledgebase, Human AOX1, entry no. Q06278, https://www.uniprot.org/uniprot/Q06278).