Enzyme | Marker Activity | Substrate Concentration | CHH | Incubation Time | Mass transition Monitored | Ionization Mode | Internal Standard |
---|---|---|---|---|---|---|---|
μM | million cells/ml | min | m/z | ||||
CYP1A2 | Phenacetin O-deethylation | 20 | 0.5 | 10 | 152/110 | ESI+ | d4-Acetaminophen |
CYP2B6 | Bupropion hydroxylation | 10 | 0.5 | 10 | 256/167 | ESI+ | d6-Hydroxybupropion |
CYP2C8 | Amodiaquine N-dealkylation | 3 | 0.5 | 10 | 328/283 | ESI+ | d3-N-Desethylamodiaquine |
CYP2C9 | Tolbutamide hydroxylation | 20 | 0.5 | 10 | 287/171 | ESI+ | d9-Hydroxytolbutamide |
CYP2C19 | S-Mephenytoin 4′-hydroxylation | 5 | 0.5 | 10 | 235/150 | ESI+ | d3-4′-Hydroxymephenytoin |
CYP2D6 | Dextromethorphan O-demethylation | 1 | 0.5 | 10 | 258/157 | ESI+ | d3-Dextrorphan |
CYP2E1 | Chlorzoxazone 6-hydroxylation | 45 | 0.5 | 10 | 184/120 | ESI– | d2-6-hydroxychlorzoxazone |
CYP3A4 | Midazolam 1′-hydroxylation | 2 | 0.5 | 10 | 342/324 | ESI+ | 13C3-1′-Hydroxymidazolam |
ESI, electrospray ionization.