TABLE 3

Cocktail incubation approach using pooled HLMs and the Tecan Fluent system

Ten UGT probe substrates were incubated either individually or in cocktails. Values are presented as the mean ± S.D. unless otherwise mentioned.

Cocktail Group	UGT Probe Substrate	UGT Isoform	Incubation Concentration	Relative Rate (CV)^{^b}		Fold Difference
Cocktail Group	UGT Probe Substrate	UGT Isoform	Incubation Concentration	Individual Incubation	Cocktail Incubation	Fold Difference
			µM	%
1	Trifluoperazine	UGT1A4	5	100 ± 3.3	111.5 ± 7.3	1.1
	Gemfibrozil	UGT2B4/2B7	5	100 ± 12.9	92.1 ± 13.6	0.9
2	Amitriptyline	UGT2B10	5	100 ± 10.7	94.2 ± 20.0	0.9
	Oxazepam^{^a}	UGT2B15	5	100 ± 2.0	104.7 ± 21.2	1.0
3	Propofol	UGT1A9	5	100 ± 13.3	105.1 ± 11.8	1.1
	Zidovudine	UGT2B7	100	100 ± 4.4	97.5 ± 4.4	1.0
4	Chenodeoxycholic acid	UGT1A3	5	100 ± 10.9	98.0 ± 7.6	1.0
	5-Hydroxytryptophol	UGT1A6	5	100 ± 12.0	101.0 ± 6.1	1.0
	Testosterone	UGT2B17	5	100 ± 3.9	87.1 ± 7.2	0.9
5	β-Estradiol	UGT1A1	5	N/A	N/A	N/A

CV, coefficient of variation; N/A, not applicable.
↵^a Incubation of racemic oxazepam.
↵^b Data are reported as the relative rate percentage (CV%) (n = 3). The relative rate was measured in the individual incubation and was defined as 100% for each substrate.