The effect of repeated injection of the 5-HT2 receptor agonist DOI into the ARC (3 μg/side) on the mRNA expression level of CYP2C11, CYP3A1/23, and CYP3A2 genes in rat liver
The results are expressed as the fold change in relation to two housekeeping genes, i.e., HPRT1 and GAPDH. All of the values are shown as the mean fold change (±S.E.M.) calculated by the comparative 2−∆∆Ct method for the control (n = 6–8) and agonist-treated (n = 6 to 7) groups. Statistical significance was assessed by Student’s t test and is indicated as P < 0.05; P < 0.01, compared with the control.
| Gene Name | HPRT1 Gene | GAPDH Gene | ||
|---|---|---|---|---|
| Fold Change | Statistical Significance | Fold Change | Statistical Significance | |
| CYP2C11 | 1.71 ↑ (±0.18) | P < 0.05 | 1.60 ↑ (±0.16) | P < 0.05 |
| CYP3A1/23 | 2.60 ↑ (±0.87) | P = 0.228 | 2.76 ↑ (±0.50) | P < 0.05 |
| CYP3A2 | 4.06 ↑ (±0.91) | P < 0.05 | 4.49 ↑ (±1.19) | P < 0.01 |