The mean values and S.E. of kinetic parameters of resorufin formation by human recombinant CYP1B1 in the absence and presence of 19(R)-HETE or 19(S)-HETE
Results are presented as mean and S.E., of at least three individual experiments. Km, Vmax and Ki mean ± S.E. were determined by the Enzyme Kinetics module of GraphPad Prism, version 5.01.
Km | Vmax | Ki | |
---|---|---|---|
19(R)-HETE | 19(S)-HETE | ||
nM | pmol/pmol P450 per minute | nM | nM |
86.6 ± 10.4 | 16.7 ± 1.1 | 89.1 ± 11.5 | 37.3 ± 5.5 |