Potency of noscapine and several chemical derivatives to elicit cytotoxicity in drug-sensitive and -resistant breast cancer cell lines
The potencies of drugs to inhibit cell proliferation (IC50) were determined using the MTT assay described in the Materials and Methods. Values (mean ± S.E.M.) were obtained by nonlinear regression of the general dose-response relationship. The values in parentheses correspond to the number of independent observations.
| Compound | Cytotoxic Potency IC50(μM) | |
|---|---|---|
| MCF7 | NCI/AdrRES | |
| Noscapine | 45.4 ± 6.5 (13) | 32.1 ± 7.0 (12) |
| (2) | NE (5) | NE (4) |
| (3) | N.E (6) | NE (4) |
| (4) | 5.3 ± 0.9* (6) | 3.3 ± 0.41* (5) |
| (5) | 1.6 ± 0.4* (11) | 6.4 ± 2.4* (4) |
| (6) | 2.5 ± 0.4* (11) | 3.0 ± 0.6* (6) |
| (7) | 14.6 ± 2.5* (8) | 12.6 ± 2.8* (7) |
| (8) | 2.5 ± 0.5* (11) | 2.1 ± 0.5* (6) |
| (9) | 2.3 ± 0.7* (15) | 2.1 ± 0.5* (7) |
| (10) | 4.3 ± 0.7* (7) | 2.4 ± 0.2* (7) |
| (11) | 21.5 ± 7.3* (6) | 18.3 ± 2.8 (3) |
MCF, human breast adenocarcinoma cell line; MTT, 3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide; NE, compound did not generate a cytotoxic response.
* Statistically significant difference (P < 0.01) compared with the value obtained for noscapine in the specific cell line.