TABLE 2

Results for model fitting to EII-IL datasets with 5% error

All four models were fit to the all the datasets with different fumic. [I] values (n = 8) were used as detailed in the table.

Simulated withParametersMM (Post Hoc)aMM-IL (Concurrent)EII (Post Hoc)aEII-IL (Concurrent)
EII-IL fumic = 0.02; KI,u1 = 0.1 µM; KI,u2 = 1 µM; kinact = 0.14 min; [I] range: 0–80 µMKI,uDNCDNCKI,u1 = 0.004 ± 0.0002
KI,u2 = 1.66 ± 0.29KI,u1 = 0.08 ± 0.006
KI,u2 = 0.81 ± 0.14
kinactDNCDNC0.006 ± 0.0010.14 ± 0.032
kinact/KI,uDNCDNCkinact/KI,u1 = 1.53kinact/KI,u1 = 1.75
% convergence0064100
Average AICcDNCDNC−587−616
Average r2DNCDNC0.9970.998
EII-IL fumic = 0.1; KI,u1 = 0.1 µM; KI,u2 = 1 µM; kinact = 0.14 min; [I] range: 0–40 µMKI,uDNC13.7 ± 10.6KI,u1 = 0.008 ± 0.0004
KI,u2 = 1.21 ± 0.14KI,u1 = 0.1 ± 0.006
KI,u2 = 0.98 ± 0.11
kinactDNC2.4 ± 1.70.02 ± 0.0040.14 ± 0.018
kinact/KI,uDNC0.39 ± 0.23kinact/KI,u1 = 2.88kinact/KI,u1 = 1.38
% convergence02676100
Average AICcDNC−398−642−681
Average r2DNC0.9130.9960.998
EII-IL fumic = 0.5; KI,u1 = 0.1 µM; KI,u2 = 1 µM; kinact = 0.14 min; [I] range: 0–40 µMKI,u0.02 ± 0.0030.04 ± 0.005KI,u1 = 0.04 ± 0.002
KI,u2 = 0.94 ± 0.10KI,u1 = 0.1 ± 0.004
KI,u2 = 1.0 ± 0.07
kinact0.15 ± 0.010.15 ± 0.010.09 ± 0.010.14 ± 0.01
kinact/KI,u8.2 ± 1.44.2 ± 0.70kinact/KI,u1 = 2.38kinact/KI,u1 = 1.40
% convergence781001498
Average AICc−529−534−803−808
Average r20.9190.9240.9970.998
EII-IL fumic = 0.9; KI,u1 = 0.1 µM; KI,u2 = 1 µM; kinact = 0.14 min; [I] range: 0–10 µMKI,u2.0 ± 0.201.98 ± 0.18KI,u1 = 0.09 ± 0.01
KI,u2 = 1.0 ± 0.08KI,u1 = 0.1 ± 0.01
KI,u2 = 1.0 ± 0.04
kinact0.38 ± 0.020.38 ± 0.020.13 ± 0.010.14 ± 0.01
kinact/KI,u0.2 ± 0.020.2 ± 0.02kinact/KI,u1 = 1.51kinact/KI,u1 = 1.40
% convergence100100100100
Average AICc−557−558−709−709
Average r20.9840.9850.9980.998
  • DNC, did not converge.

  • a KI,u was calculated as KI ·fumic.