mRNA expression of CES1 and CES2 in Caco-2 and HEK293-OATP2B1 cells
ΔCt = Ct of CES − Ct of actin. NA: The mRNA ratio for CES1 in HEK293-OATP2B1 cells was not calculated, as the CES1 mRNA levels were very low.
| Cells | mRNA | ΔCt Values | mRNA Ratio (B/A) | |
|---|---|---|---|---|
| Nontransfected (A) | CES2-Transfected (B) | |||
| Caco-2 | CES1 | 0.78 ± 0.15 | 0.65 ± 0.15 | 1.09 ± 0.01 |
| CES2 | 10.5 ± 0.2 | 2.96 ± 0.17 | 194 ± 29 | |
| HEK293-OATP2B1 | CES1 | 12.0 ± 0.6 | 16.4 ± 1.9 | NA |
| CES2 | 8.27 ± 0.06 | 2.4 ± 0.2 | 58.5 ± 7.6 | |