SRM parameters of peptides used for targeted analysis of hepatobiliary transporters (Oatp1a1, Oatp1a4, Oatp1b2, Ntcp, Mrp3, Mrp2, Bsep, Na+/K+ ATPase) for LC-MS/MS–based proteomic analysis in WT and PCK rat livers. The labeled amino acid residue of the internal standard is shown with an asterisk.
| Gene/Protein | Probe Sequence | SRM Transition | |||
|---|---|---|---|---|---|
| Parent Ion (Q1) | Product Ions (Q3) | ||||
| 1 | 2 | 3 | |||
| Slco1a1/Oatp1a1 | EENLGITK | 452.2 | 645.4 | 531.4 | 418.3 |
| EENLGITK* | 456.2 | 653.4 | 539.4 | 426.3 | |
| Slco1a4/Oatp1a4 | TFQFPGDIESSK | 678.4 | 832.4 | 735.4 | 563.4 |
| TFQFPGDIESSK* | 682.4 | 840.4 | 743.4 | 571.4 | |
| Slco1b2/Oatp1b2 | SVQPELK | 400.8 | 614.4 | 486.3 | 260.2 |
| SVQPELK* | 404.8 | 622.4 | 494.3 | 268.2 | |
| Slc10a1/Ntcp | AAATEDATPAALEK | 679.9 | 729.4 | 915.5 | 628.4 |
| AAATEDATPAALEK* | 683.9 | 737.4 | 923.5 | 636.4 | |
| Abcc3/Mrp3 | FYVATSR | 422.4 | 696.4 | 533.3 | 434.2 |
| FYVATSR* | 427.4 | 706.4 | 543.3 | 444.2 | |
| Abcc2/Mrp2 | LTIIPQDPILFSGSLR | 885.5 | 989.6 | 1329.7 | 666.4 |
| LTIIPQDPILFSGSLR* | 890.5 | 999.6 | 1339.7 | 676.4 | |
| Abcb11/Bsep | STSIQLLER | 523.8 | 530.3 | 658.4 | 858.5 |
| STSIQLLER* | 528.8 | 540.3 | 668.4 | 868.5 | |
| Na+/K+ ATPase/Na+/K+ ATPase | AAVPDAVGK | 414.2 | 685.4 | 586.3 | 374.2 |
| AAVPDAVGK* | 418.2 | 693.4 | 594.3 | 382.2 | |