Ion mode application, mass transitions, and linearity range used for the quantification of marker metabolites of the isoform-specific pathways used for kinetic experiments in MMHHs and inhibition experiments in both MMHHs and CHHs
| P450 Isozyme | Marker Substrate | Marker Metabolite | Ion Mode | Mass Transition | Linearity Range |
|---|---|---|---|---|---|
| m/z | nM | ||||
| CYP1A2 | Phenacetin | Acetaminophen | Positive | 152.2→110 | 6.6–6622.5 |
| CYP2A6 | Coumarin | Hydroxy coumarin | Negative | 160.8→132.7 | 6.2–6211.2 |
| CYP2B6 | Bupropion | Hydroxy bupropion | Positive | 256→139 | 3.9–3921.6 |
| CYP2C8 | Amodiaquine | Desethyl amodiaquine | Positive | 328.3→283.3 | 3.1–3058.1 |
| CYP2C9 | Diclofenac | Hydroxy diclofenac | Positive | 311.927→266 | 3.2–3215.4 |
| CYP2C19 | Mephenytoin | Hydroxy mephenytoin | Positive | 235.1→150.2 | 4.3–4273.5 |
| CYP2D6 | Dextromethorphan | Dextrorphan | Positive | 258.3→157.2 | 3.9–3891.1 |
| CYP2E1 | Chlorzoxazone | Hydroxy chlorzoxazone | Negative | 184→120.1 | 5.4–5434.8 |
| CYP3A4 | Midazolam | Hydroxy midazolam | Positive | 342→203 | 2.9–2932.6 |
| CYP3A4 | Testosterone | Hydroxy testosterone | Positive | 305.1→269.1 | 3.9–3289.5 |