Summary of MRP2 and MRP3 vesicle kinetic studies for DCF-AG
MRP2 had single-Km transport affinity for DCF-AG, whereas MRP3 demonstrated biphasic kinetics. Intrinsic transporter clearance (Vmax/Km) for MRP2 and the low-affinity MRP3 clearance was comparable. In contrast, the high-affinity MRP3 efflux clearance was over an order of magnitude greater than that of MRP2.
| Transporter | Vmax | Km | Transport CLint |
|---|---|---|---|
| pmol/min/mg | µM | µl/min/mg | |
| MRP2 | 130 | 50.5 | 2.58 |
| MRP3 (low affinity) | 170 | 98.2 | 2.37 |
| MRP3 (high affinity) | 71.9 | 1.78 | 40.3 |
CLint, intrinsic uptake clearance.