TABLE 2

Validation of 50 kDa MWMCO ultrafiltration method for determination of fu,plasma and fu,liver using well characterized small molecules

SMMatrix (Human)fu (Measured)fu (Reference)Reference Method
AntipyrinePlasma0.867 ± 0.1100.9Ultracentrifugation
TimololPlasma0.347 ± 0.0740.4Ultracentrifugation
WarfarinPlasma0.043 ± 0.0070.01aUltracentrifugation
RosuvastatinLiver homogenate0.14 ± 0.030.23bEquilibrium dialysis

  • SM, small molecule.

  • ↵a O’Reilly (1972).

  • ↵b Pfeifer et al. (2013); Yoshikado et al. (2017).