Pharmacokinetic parameters of m1A and rhodamine 123 in control and PYR-treated mice
The equations to calculate the kinetic parameters are described in Materials and Methods. Each value represents the mean ± S.E. (n = 4); Student’s two-tailed unpaired t test; value in control mice vs. PYR-treated mice.
| Parameter | m1A | Rhodamine 123 | ||||
|---|---|---|---|---|---|---|
| Control | PYR | Unit | Control | PYR | Unit | |
| Cp,120 min | 1.64 ± 0.10 | 2.31 ± 0.13** | μM | 5.61 ± 0.46 | 5.01 ± 0.39 | nM |
| AUCp,0–120 min | 140 ± 9 | 182 ± 11* | μmol per min/l | 564 ± 27 | 512 ± 12 | nmol per min/l |
| Xurine | 170 ± 10 | 126 ± 5** | nmol | 477 ± 32 | 21.2 ± 3.4*** | pmol |
| Ckidney | 7.22 ± 1.82 | 34.0 ± 5.4** | μM | 0.800 ± 0.074 | 1.86 ± 0.21 | μM |
| Kp,kidney | 4.4 ± 1.1 | 14.7 ± 2.0** | ml/g of kidney | 143 ± 6 | 381 ± 70 | ml/g of kidney |
| CLtot,p | 2.32 ± 0.12 | 1.78 ± 0.13* | ml/min | 3.93 ± 0.18 | 4.07 ± 0.05 | ml/min |
| CLtot,p | 80.4 ± 4.9 | 61.9 ± 3.6* | ml/min per kilogram | 136 ± 8 | 142 ± 2 | ml/min per kilogram |
| CLr,p | 1.23 ± 0.11 | 0.698 ± 0.035** | ml/min | 0.851 ± 0.073 | 0.0416 ± 0.0073 | ml/min |
| CLr,p | 42.6 ± 4.1 | 24.3 ± 0.6** | ml/min per kilogram | 29.6 ± 3.1 | 1.44 ± 0.21 | ml/min per kilogram |
| GFR (Jonker et al., 2003) | 0.462 ± 0.045 | 0.462 ± 0.065 | ml/min | |||
Ckidney, kidney concentration; Cp,120 min, plasma concentration of compound at 120 minutes after administration; GFR, glomerular filtration rate; Xurine, amount of compound excreted in urine.
↵* P < 0.05; **P < 0.01; ***P < 0.001.