Comparison of CYP and accessory protein (P450 oxidoreductase, POR; cytochrome B5A, CYB5A) abundance in liver microsomes from four different dog breeds and 13 mixed-breed dogs
Shown are the median and interquartile range (25%–75%) for each breed and mixed-breed dogs and the results of ANOVA on rank-transformed data. In instances where ANOVA results were significant (P < 0.05), Dunn’s test was used to isolate breed groups that were significantly different from each other in the abundance of that protein.
| Liver Microsome Abundance [Median (25%–75%) pmol/mg Protein] | |||||||
|---|---|---|---|---|---|---|---|
| Protein | Research Hounds (N = 12) | Research Beagles (N = 25) | Mixed-Breed Dogs (N = 13) | Chihuahuas (N = 4) | Greyhounds (N = 5) | ANOVA (P Value) | Dunn’s Test (P < 0.05) |
| CYP1A1 | 0.2 (0.0–1.0) | 0.0 (0.0–3.9) | 1.6 (0.2–4.9) | 1.6 (1.0–2.4) | 0.8 (0.2–1.5) | 0.39 | — |
| CYP1A2 | 53 (42–66) | 72 (54–107) | 61 (37–98) | 65 (43–85) | 48 (44–74) | 0.35 | — |
| CYP2A13 | 13 (11–16) | 8.6 (5.6–13.5) | 6.5 (4.3–9.3) | 9.3 (3.4–16.1) | 16 (15–17) | 0.043 | — |
| CYP2A25 | 5.4 (4.1–6.0) | 8.2 (6.5–11.2) | 9.8 (6.8–10.8) | 11.6 (7.9–14.5) | 6.8 (4.8–7.6) | 0.008 | — |
| CYP2B11 | 120 (112–129) | 78 (67–101) | 45 (37–72) | 40 (37–47) | 30 (28–31) | <0.0001 | a,b,c,d |
| CYP2C21 | 40 (36–44) | 58 (50–77) | 50 (47–56) | 48 (43–54) | 47 (39–48) | 0.006 | e |
| CYP2C41f | 7.8 (7.1–10.3) | 5.0 (2.0–9.4) | 9.7 | 6.2 | — | 0.37 | — |
| CYP2D15 | 167 (140–186) | 127 (100–181) | 138 (112–148) | 136 (115–167) | 131 (118–135) | 0.16 | — |
| CYP2E1 | 68 (59–76) | 72 (59–84) | 67 (62–80) | 72 (53–88) | 97 (81–99) | 0.35 | — |
| CYP3A12 | 145 (130–174) | 118 (108–139) | 93 (70–127) | 117 (107–132) | 91 (87–144) | 0.031 | a |
| CYP3A26 | 5.2 (4.5–6.2) | 3.0 (2.2–4.3) | 3.2 (2.6–5.2) | 3.1 (2.2–3.9) | 2.1 (0.6–3.0) | 0.002 | c |
| POR | 40 (36–42) | 42 (33–49) | 41 (28–51) | 39 (36–43) | 32 (30–35) | 0.44 | — |
| CYB5A | 436 (393–458) | 464 (425–506) | 402 (381–429) | 427 (380–442) | 382 (328–459) | 0.09 | — |
↵a Research hounds vs. mixed-breed dogs.
↵b Research hounds vs. Chihuahuas.
↵c Research hounds vs. greyhounds.
↵d Research Beagles vs. greyhounds.
↵e Research hounds vs. research Beagles.
↵f Note that the results given for CYP2C41 are for the 12 dogs that express at least one copy of the CYP2C41 gene (six research hounds, four Beagles, one mixed-breed, and one Chihuahua).