TABLE 1

Metabolic activity of nasal mucosa, lung, and liver microsomes of CYP2A13/2F1-humanized-and-LCN and Cyp2abfgs-null-and-LCN mice in vitro

Rates of NA-GSH formation were determined as described in the Materials and Methods. Values represent means ± S.D. (n = 3 to 4).

Tissue	Rate of NA-GSH formation
Tissue	CYP2A13/2F1-humanized-and-LCN	Cyp2abfgs-null-and-LCN	CYP2A13/2F1-humanized^{^a}	Cyp2abfgs-null^{^a}	LCN^{^b}
	pmol/min per mg microsomal protein
Nasal mucosa	801 ± 77	206 ± 6^{^c}	964 ± 59	118 ± 12	N.A.
Lung	19.9 ± 4.9	11.9 ± 0.5^{^d}	23.1 ± 3.2	8.1 ± 0.5	1120 ± 198
Liver	76.9 ± 13.5	94.7 ± 26.3	N.A.	N.A.	83.7 ± 8.7

Reaction mixtures contained 50 mM phosphate buffer (pH 7.4), 10 mM GSH, 1 mM NADPH, various concentrations of microsomal proteins (0.2 mg/ml for individual livers, 0.25 mg/ml for pooled olfactory mucosa, and 1 mg/ml for pooled lung), and 100 µM NA. Statistical analysis was performed using two-way ANOVA followed by the Bonferroni test for multiple comparisons. Previously reported data for LCN, Cyp2abfgs-null, and CYP2A13/2F1-humanized mice (Kovalchuk et al., 2017; Li et al., 2017) are shown for comparison. N.A., not analyzed.
↵^a Extracted from Figure 1 of Li et al. (2017) for rates at 100 µM NA.
↵^b From original data used for kinetic analysis in Table 1 of Kovalchuk et al. (2017) for rates at 100 µM NA.
↵^c P < 0.0001, compared with CYP2A13/2F1-humanized-and-LCN mice.
↵^d P < 0.05, compared with CYP2A13/2F1-humanized-and-LCN mice.