Effect of menadione on human liver microsome metabolism of oxycodone N-oxide
Values are expressed as means ± S.D., mean of four determinations, pH 7.4. Oxycodone N-oxide was present at 20 µM. Incubations were conducted for 30 min.
| Description | Oxycodone | Oxycodone N-Oxide | Noroxycodone | Oxymorphone | Noroxymorphone |
|---|---|---|---|---|---|
| nmol/mg protein | |||||
| Complete | 0.06 ± 0.02 | 0.2 ± 0.01 | 0.01 ± 0.003 | 0.001 ± 0.0001 | ND |
| −NADPH | 0.01 ± 0.003 | 0.2 ± 0.04 | 0.001 ± 0.001 | ND | ND |
| +NADPH +Menadione | 0.01 ± 0.001 | 0.3 ± 0.02 | 0.001 ± 0.0001 | ND | ND |
| −NADPH +Menadione | 0.01 ± 0.002 | 0.3 ± 0.03 | 0.0004 ± 0.0004 | ND | ND |
ND, not detected.