Retro-reduction of oxycodone N-oxide by human liver microsomes with or without heat inactivation
Values are expressed as means ± S.D., mean of four determinations, pH 7.4.
| Human Liver Microsomes HK37a | Time | Oxycodone | Oxycodone N-Oxide | Noroxycodone | Oxymorphone | Noroxymorphone |
|---|---|---|---|---|---|---|
| min | (nmol/ml)/mg protein | nmol/mg | (nmol/ml)/mg protein | |||
| No heat treatment | 0 | 2.5 ± 0.2 | 11.8 ± 0.5 | 0.3 ± 0.02 | ND | ND |
| 20 | 4.4 ± 0.2 | 11.3 ± 2.0 | 0.6 ± 0.1 | ND | ND | |
| Heat treatment | 0 | 2.9 ± 0.4 | 12.0 ± 1.3 | 0.4 ± 0.06 | ND | ND |
| 20 | 3.5 ± 0.3 | 15.8 ± 5.2 | 0.6 ± 0.2 | ND | ND | |
ND, not detected.
↵a Oxycodone N-oxide was present at 20 µM.