N-Oxygenation of selective functional substrates by mouse and human FMOs
| Substrate | mFMO1 | hFMO1 | mFMO3 | hFMO3 | mFMO5 | hFMO5 |
|---|---|---|---|---|---|---|
| (nmol product/min)/mg of protein | ||||||
| 3-DPT | 195 ± 18 | 20 ± 1 | 2 ± 0 | 6 ± 0 | ND | NA |
| 5-DPT | 407 ± 21 | 35 ± 3 | 41 ± 3 | 34 ± 1 | 1.5 ± 0 | NA |
| 8-DPT | 294 ± 35 | 24 ± 2 | 69 ± 1 | 55 ± 8 | 11 ± 0 | 7 ± 0 |
DPT, 10-(dimethylamino alkyl)-(3-trifluoromethyl)-phenothiazine, where 3-DPT, 5-DPT, and 8-DPT has a 3-carbon, 5-carbon, and 8-carbon alkyl group, respectively; h, human; m, mouse; NA, not attempted; ND, not detectable.