Dithiolthiones | C.S.a | C.S. − NADPHb | C.S. + Bz-ImHc | C.S. + GSHd |
---|---|---|---|---|
Ph-DT | 7.5 ± 2.7 | 2.0 ± 0.2 | 3.6 ± 1.1 | 8.5 ± 1.0 |
ADT | 12.0 ± 3.5 | 3.1 ± 1.3 | 2.9 ± 1.0 | 6.4 ± 1.6 |
dmADT | 2.5 ± 1.0 | 2.9 ± 1.7 | 2.5 ± 1.9 | 5.5 ± 1.8 |
p-Cl-Ph-DT | 7.0 ± 1.1 | 2.4 ± 2.3 | 2.0 ± 0.8 | 3.3 ± 1.3 |
o-Cl-Ph-DT | 6.0 ± 0.3 | 1.2 ± 0.3 | 1.5 ± 1.0 | 1.8 ± 0.8 |
o-OH-ADT | 12.4 ± 2.5 | 3.5 ± 1.5 | 3.0 ± 0.8 | 9.3 ± 1.0 |
Oltipraz | 5.7 ± 3.0 | 1.5 ± 0.5 | 1.9 ± 1.4 | 1.5 ± 1.7 |
Naproxene-dmADT | 6.3 ± 0.7 | 2.3 ± 1.8 | 4.5 ± 1.0 | 4.7 ± 0.7 |
Aspirine-dmADT | 5.1 ± 1.0 | 4.5 ± 0.7 | 2.2 ± 1.1 | 7.4 ± 3.1 |
↵a H2S yields (%) were measured after 1 h of incubation of 100 µM dithiolethione in phosphate buffer (pH 7.4) in the presence of liver microsomes (∼2.5 µM cytochrome P450) and 1 mM NADPH, as described in Materials and Methods. Data are yields based on starting compound and are means ± S.D. from three to eight experiments.
↵b Identical conditions but in the absence of NADPH.
↵c Identical conditions, but 1 mM Bz-ImH was added in the solution.
↵d Identical conditions but in the presence of 1 mM GSH.
C.S., complete system.