TABLE 1

Kinetic parameters (mean ± S.D.) describing metabolism of amodiaquine and midazolam in HLM suspensions and HLM-MGBS

ParameterAmodiaquine (CYP2C8)Midazolam (CYP3A)
HLMSIHLM-MGBSSIHLMMMHLM-MGBSSI
Km (µM)0.732 ± 0.111.21 ± 0.08ns0.612 ± 0.0862.04 ± 0.23sig
Vmax (pmol/min per milligram)982 ± 891030 ± 87ns2002 ± 664350 ± 249sig
CLint (µl/min per milligram)134185128282132
  • MM, Michaelis-Menten modeling used; ns, not significantly different from parameter generated using HLM; SI, substrate inhibition modeling used; sig, significantly different from parameter generated using HLM (P < 0.05).