TABLE 2

Kinetic parameters (mean ± S.D.) of UGT1A1, UGT1A9, and UGT2B7 in HLM suspensions and HLM-MGBS

ParameterEstradiol (UGT1A1)ParameterPropofol (UGT1A9)AZT (UGT2B7)
HLMASHLM-MGBSASHLMSIHLM-MGBSSIHLMMMHLM-MGBSMM
S50 (µM)13 ± 16.8 ± 0.6sigKm (µM)30 ± 413 ± 2sig1400 ± 0.1490 ± 0.06sig
Vmax (pmol/min per milligram)761 ± 34600 ± 25sigVmax (pmol/min per milligram)2029 ± 491635 ± 66sig614 ± 211366 ± 42sig
CLint (µl/min per milligram)5988CLint (µl/min per milligram)681260.442.8
  • AS, allosteric sigmoidal modeling used; MM, Michaelis-Menten modeling used; ns, not significantly different from parameter generated using HLM; SI, substrate inhibition modeling used; sig, significantly different from parameter generated using HLM (P < 0.05).