TABLE 2

Drug metabolizing enzyme activities (picomoles per minute per milligram protein) in CHIM

Means and S.E.s (S.E.M.) of CHIM from four donors are shown. The results are derived from those previously published (Li et al., 2018a).

Drug metabolizing enzyme	Substrate	Substrate conc. (µM)	Marker metabolite	Mean	S.E.M.
CYP1A1	7-Ethoxyresozufin	20	Resozufin	0.26	0.13
CYP1A2	Phenacetin	100	Acetaminophen	3.34	0.89
CYP2A6	Coumarin	50	7-HC, 7-HC-sulfate, 7-HC-glucuronide	NA	NA
CYP2B6	Buproprion	500	Hydroxybuproprion	0.69	0.11
CYP2C8	Paclitaxel (taxol)	20	6α-Hydroxypaclitaxel	0.12	0.02
CYP2C9	Diclofenac	25	4-OH diclofenac	0.39	0.05
CYP2C19	S-mephenytoin	250	4-OH S-mephenytoin	0.86	0.26
CYP2D6	Dextromethorphan	15	Dextrophan	0.04	0.02
CYP2E1	Chlorzoxazone	250	6-OH chlorzoxazone	0.04	0.01
CYP2J2	Astemizole	50	O-demethyl astemizole	2.27	0.31
CYP3A4-1	Midazolam	20	1-Hydroxymidazolam	1.83	0.65
CYP3A4-2	Testosterone	200	6β-Hydroxytestosterone	24.82	5.47
ECOD	7-Ethoxycoumarin	100	7-HC, 7-HC-sulfate, 7-HC-glucuronide	0.81	0.24
UGT	7-Hydroxycoumarin	100	7-Hydroxycoumarin glucuronide	0.71	0.26
SULT	7-Hydroxycoumarin	100	7-Hydroxycoumarin sulfate	1.85	0.44
GST	Acetaminophen	10 mM	Acetaminophen glutathione	0.26	0.09
UGT	Acetaminophen	10 mM	Acetaminophen glucuronide	1.04	0.51
SULT	Acetaminophen	10 mM	Acetaminophen sulfate	4.52	0.84
FMO	Benzydamine HCl	250	Benzydamine-N-oxide	8.11	0.99
MAO	Kynuramine HBr	160	4-Hydroxyquinoline	317.09	80.12
AO	Cabazeran	20	4-Hydroxycabazeran	0.02	0.00
NAT1	4-Aminobenzoic acid	200	N-acetyl-p-aminobenzoic acid	2.17	0.58
NAT2	Sulfamethazine	100	N-acetyl-sulfamethazine	1.46	0.16
CES2	Irinotecan	50	SN38	1.21	0.13