TABLE 1

The protein binding of DHC with mouse plasma and heart (means ± S.D., n = 3) is presented

The protein binding of DHC with the concentrations of 0.1–10 μM were performed by an ultrafiltration method. The protein binding was determined as the protein binding (%) = 1−Cf/Ct × (1+P%), where Cf was the free concentration, Ct was the total concentration, and P% was the nonspecific binding of the centrifugal ultrafiltration device.

Protein Binding (%)
PlasmaMeanHeartMean
0.1 μM89.66 ± 1.988.50 ± 1.066.72 ± 2.1
DHC1 μM88.17 ± 2.449.73 ± 8.458.89 ± 8.6
10 μM87.67 ± 1.160.21 ± 2.2
Quinidine (10 μM)74.01 ± 2.0NA