TABLE 2

Abundance of [14C]TAC catabolites in urine and feces after single intravenous administration of [14C]TAC in rats

AnalytesProtonated Molecular FormulaTheoretical m/z for [M+H]+Measured m/z for [M+H]+aMass Accuracy (ppm)Percentage of ID in FecesPercentage of ID in Urine
C1NANANANAND8.34
C2NANANANAND4.93
C3bC4714CH61N4O12+887.4313887.4292−2.3717.920.24
C4bC4614CH57N4O11+855.4051855.4028−2.69
C5cC4814CH61N4O13+915.4262915.42862.62NA0.25
C6dC4814CH59N4O12+897.4156897.4138−2.0141.360.32
C7dC4914CH63N4O13+929.4419929.4396−2.47
  • ID, injected dose; NA, not applicable; ND, not detected.

  • a The m/z values reported for dmDNA31 and its metabolites were from the most abundant ion incorporating one 14C-.

  • b Catabolites C3 and C4 share the same retention time.

  • c C5 was identified as the N-desmethyl-dmDNA31 metabolite, so it was observed at m/z 913.4218 and m/z 915.4286 at an approximately 1:1 ratio, resulting from the loss of either the 14C-labeled methyl or nonlabeled methyl, respectively.

  • d Catabolites C6 and C7 share the same retention time.