A comparison between the rate of product formation between the two species as well as the mutants under anaerobic conditions using a glucose oxidase system as described in the Materials and Methods section.
MAM was the model of choice in fitting the O6BG (200 μM) product formation for hAO and all the mutants and Michaelis-Menten linear model was used to fit the O6BG product formation in mAO as well as the product formation for dantrolene (30 μM) in both species and the mutants.
| Rate of 8-oxo-O6BG formation | Rate of ADNTN formation | |||
|---|---|---|---|---|
| k3_ox | k4 | k5 | k3_red | |
| min−1 | min−1 | min−1 | min−1 | |
| hAO | 110 ± 20.7 | 0.160 ± 0.0494 | 9.28 ± 2.03 | 0.659 ± 0.0182 |
| mAO | 2.19 ± 0.0696 | — | — | 0.156 ± 0.00876 |
| F885L | 38.7 ± 3.85 | 0.0388 ± 0.0144 | 7.90 ± 2.71 | 0.701 ± 0.0193 |
| V811A | 40.7 ± 8.51 | 0.158 ± 0.0486 | 2.95 ± 0.522 | 0.336 ± 0.0143 |
| FLVA | 30.4 ± 5.02 | 0.0659 ± 0.0289 | 7.50 ± 1.37 | 0.486 ± 0.0199 |