TABLE 1

The human to monkey (hAO#mAO) mutants were made using the primers, DNA templates, and the sequencing primers as described

Plasmid (hAO#mAO)PrimerDNA templateSequencing primer
F885LFW: 5′-cag​acc​cat​ttc​aat​aac​taa​cag​gct​ttc​atc​cag​g-3′pTHco-AOX15′-att​gtt​gcc​agc​aca​ctg​aa-3′ (nucleotides 2401–2800)
RV: 5′-cct​gga​tga​aag​cct​gtt​agt​tat​tga​aat​ggg​tct​g-3′
V811AFW: 5′-aat​aat​acc​ggt​ttt​cag​cgc​ttt​acc​acc​aaa​tgc​acc-3′pTHco-AOX1
RV: 5′-ggt​gca​ttt​ggt​ggt​aaa​gcg​ctg​aaa​acc​ggt​att​att-3′
FLVAFW: 5′-aat​aat​acc​ggt​ttt​cag​cgc​ttt​acc​acc​aaa​tgc​acc-3′F885L
RV: 5′-ggt​gca​ttt​ggt​ggt​aaa​gcg​ctg​aaa​acc​ggt​att​att-3′