The change in the ratio of the concentration of the oxidative to reductive product (OAR) with time under anaerobic conditions (as described in the Materials and Methods section) and saturating amount of the oxidative and reductive substrates, O6BG (200 μM) and dantrolene (30 μM), respectively
| Time, min | [8-oxo-O6BG]/[ADNTN] | ||||
|---|---|---|---|---|---|
| hAO | mAO | F885L | V811A | FLVA | |
| μM/μM | μM/μM | μM/μM | μM/μM | μM/μM | |
| 0.2 | 55.6 ± 19.3 | 4.41 ± 0.622 | 86.0 ± 42.2 | 46.1 ± 23.9 | 21.7 ± 4.54 |
| 3 | 71.8 ± 26.8 | 3.59 ± 1.38 | 39.8 ± 8.20 | 34.4 ± 9.70 | 28.8 ± 1.54 |
| 10 | 71.3 ± 16.9 | 4.43 ± 1.32 | 42.1 ± 7.22 | 39.8 ± 8.45 | 39.5 ± 2.71 |
| 20 | 61.0 ± 12.0 | 5.99 ± 0.881 | 47.2 ± 2.31 | 37.4 ± 8.09 | 40.3 ± 2.51 |
| 30 | 52.7 ± 5.68 | 5.41 ± 1.48 | 43.4 ± 1.34 | 33.8 ± 4.41 | 40.6 ± 3.53 |
| 45 | 39.3 ± 8.13 | 6.78 ± 1.64 | 38.6 ± 3.67 | 27.7 ± 2.54 | 38.1 ± 5.25 |
| 60 | 33.5 ± 5.94 | 7.63 ± 1.72 | 34.6 ± 3.24 | 23.9 ± 1.79 | 34.8 ± 6.57 |
| 90 | 25.3 ± 3.30 | 7.90 ± 1.60 | 26.7 ± 2.40 | 19.5 ± 1.09 | 29.5 ± 4.25 |
| 120 | 20.6 ± 2.57 | 8.53 ± 2.62 | 22.3 ± 2.71 | 16.3 ± 0.875 | 21.4 ± 0.972 |
| Average | 50.3 ± 20.8 | 5.83 ± 1.83 | 42.3 ± 18.2 | 31.0 ± 9.85 | 32.8 ± 7.70 |