LDH release after exposure to PHD2 inhibitors in human hepatocytes
The total amount of LDH released into the medium for 72 h from cells treated with tamoxifen (50 μM, a positive control for cell toxicity) was regarded as equivalent to 100% LDH in cells.
| Compound | Conc. (μM) | LDH Release (%)a | ||
|---|---|---|---|---|
| 0–24 h | 24–48 h | 48–72 h | ||
| Control (0.1% DMSO) | — | 3.9 | 2.9 | 1.7 |
| 2 | 10 | 3.7 | 3.0 | 2.5 |
| 3 | 10 | 3.0 | 2.0 | 2.5 |
| 4 | 10 | 3.6 | 2.0 | 1.9 |
| 5 | 10 | 3.7 | 5.7 | 6.3 |
| 6 | 30 | 3.2 | 2.0 | 2.9 |
↵a Each value represents the mean (n = 3).